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tris based buffer (Vector Laboratories)

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Vector Laboratories tris based buffer
Tris Based Buffer, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 3165 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tris+based+buffer/Antigen+Unmasking+Solution%2C+Tris-Based/bio_rxiv__64898__2026__03__24__711280-254-28-33
Average 96 stars, based on 3165 article reviews

tris based buffer - by Bioz Stars, 2026-10

96/100 stars

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Incubation:

Article Title: Development and Validation of a New BAG-1L-Specific Antibody to Quantify BAG-1L Protein Expression in Advanced Prostate Cancer.
Article Snippet: IHC at the UW used the rabbit monoclonal antieBAG-1L (RevMAb Biosciences, RM310) antibody and was performed by hand. .. TMAs were first deparaffinized and rehydrated, then heated in Tris-based buffer (Vector Laboratories, H-3301-250) in the pressure cooker to accomplish antigen unmasking before antieBAG-1L antibody (1:500) was incubated with tissue overnight at 4 C. After washes, the 3,30-diaminobenzidine based-chromogenic detection was performed using biotinylated secondary antibodies in conjunction with VECTASTAIN ELITE ABC Horseradish Peroxidase Detection Kit (Vector Laboratories, PK-6100) and ImmPACT DAB Substrate Kit, Peroxidase (horseradish peroxidase) (Vector Laboratories, SK-4105). ..

Formalin-fixed Paraffin-Embedded:

Article Title: A Compound that Inhibits Glycolysis in Prostate Cancer Controls Growth of Advanced Prostate Cancer
Article Snippet: .. The resulting 731 FFPE (formalin-fixed, paraffin-embedded) samples were sectioned at 10 μm, mounted 732 on slides, deparaffinized, rehydrated, heated up in Tris-based buffer (Vector 733 Laboratories, Cat# H-3301-250) in the pressure cooker to accomplish antigen 734 unmasking. ..

Article Title: Pancreatic expression of CPT1A is essential for whole body glucose homeostasis by supporting glucose-stimulated insulin secretion
Article Snippet: For IF staining, the following primary antibodies were used: Guinea Pig anti-insulin (Abcam Cat # ab7842 1:500), Rabbit anti-MafA (Bethyl Laboratories Cat # IHC-00352 1:50), Mouse anti-Nkx6.1 (Developmental Studies Hybridoma Bank Cat #F55A10 1:200), Rabbit Anti-GLUT2 (Proteintech Cat # 20436-1-AP 1:500), Mouse anti-Glucagon (eBioscience Cat # 14-9743-82 1:400), Rabbit Anti-ALDH1A3 (Novus Bio Cat # NBP2-15339 1:200), Rabbit anti-Ki67 (Fortis Life Sciences Cat # IHC-00375 1:300), and Rabbit anti-cleaved caspase 3 (Cell Signaling Technology Cat # 9664 1:300). .. FFPE sections were dewaxed and subjected to heat-induced epitope retrieval (20 min at 100 °C, Biocare Medical Decloaking Chamber) with either a pH 6.0 citrate [ALDH1A3, glucagon, Ki-67] or pH 9.0 tris-based buffer [MafA, Nkx6.1] (H-3300, H-3301; Vector Laboratories). .. Prior to blocking with casein (Cat # 37583; Thermo Fisher Scientific) and incubation with primary antibodies overnight at 4 °C, slides to be stained for MafA and Nkx6.1 were incubated with a 3% H 2 O 2 /TBST solution for 10 min at RT to block endogenous peroxidases.

other:

Article Title: Cell-of-Origin, not Oncogenic Effect, Determines Desmoplastic Immune Exclusion in KRAS-Driven Liver Cancer
Article Snippet: For fixation of organoids, upon aspiration of culture medium, BME was removed by incubation with cell recovery solution for 15 min on ice (Corning, #354253).